Record ID No. |
111 |
Author(s) |
Wubet T., Weiss M., Kottke I., Teketay D., Oberwinkler F. , 2004 |
Affiliation |
University of Tubingen, Institute of Botony, Morgenstelle 1, D-72076 Tubingen, Germany |
Title |
Molecular diversity of arbuscular mycorrhizal fungi in Prunus africana, an endangered medicinal tree species in dry Afromontane forests of Ethiopia |
Source. Vol.(no):Page |
New phytologist.161(2): 517-528p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Ecology Genetics |
Host |
Medicinal plants |
Organism |
Glomeromycota, G. etunicatum, G. mosseae |
Country |
Germany, Europe |
Abstracts |
The molecular diversity of arbuscular mycorrhizal (AM)
fungi colonizing roots of Prunus africana and of AM fungal
spores obtained from baiting cultures of indigenous soils from
two dry afromontane forests of Ethiopia was investigated.The
internal transcribed spacer (ITS) rDNA region from colonized
roots and single spores of three AM fungal spore types was
amplified, cloned and sequenced using AM fungal specific
primers.Phylogenetic analysis using the 5.8S rDNA data set
revealed that 109 of the sequences obtained belong to members of
the Glomeromycota. Subsequent 5.8S/ITS2 rDNA sequence analysis
indicated high AM fungal diversity and dominance of Glomus
types. Twenty sequence types belonged to the Glomeraceae and one
each to the Diversisporaceae and Archaeosporaceae. Two of the
three spore types were identified as Glomus etunicatum and
Glomus mosseae.Twenty of the AM fungal types identified are new
to Ethiopia and to science. The AM fungal community differed
between the two sites studied. C1 Univ Tubingen, Inst Bot,
D-72076 Tubingen, Germany
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