Record ID No. |
1234 |
Author(s) |
Guerin-Laguette,A., Matsushita,N., Lapeyrie,F., Shindo,K., Suzuki,K , 2005 |
Affiliation |
Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan. |
Title |
Successful inoculation of mature pine with Tricholoma matsutake |
Source. Vol.(no):Page |
Mycorrhiza. 15(4): 301-305p. |
Categories |
Ectomycorrhiza |
Subjects |
Soil plant relations |
Sub-subjects |
Plantations |
Host |
Pinus densiflora |
Organism |
Tricholoma matsutake |
Country |
Japan, Asia |
Abstracts |
Our finding demonstrates, for the first time, that the roots of mature pine trees can be successfully inoculated with a symbiotic ectomycorrhizal fungus, the valuable matsutake mushroom. Long root segments (ca. 5-10 mm in diameter, ca. 50 cm in length) of 50-year-old Pinus densiflora trees were excavated, washed, auxin-treated (2-5 mg indole butyric acid, IBA, per root) and incubated in moist Spagnum moss. Twelve months later, short roots were regenerated, of which approximately 90% were free of mycorrhizae. Mycorrhiza-free short roots were inoculated with mycelial pieces of Tricholoma matsutake and incubated further in a sterilized substrate. Four-and-a-half months later, roots putatively colonized by Matsutake were sampled near the inoculation points. A T. matsutake-specific ITS-rDNA fragment was amplified by nested PCR from approximately 80% of the root samples analyzed, whereas approximately 66% of the root samples processed for staining with Chlorazol black E displayed characteristic T. matsutake Hartig net structures. These results confirm the symbiotic infection of mature P. densiflora roots by matsutake. |