Record ID No. |
1293 |
Author(s) |
Chang DCN, Chou LC , 2007 |
Affiliation |
Chang, DCN, Natl Taiwan Univ, Dept Hort, Taipei 10617, Taiwan, E-mail: hyha@ntu.edu.tw |
Title |
Growth responses, enzyme activities, and component changes as influenced by Rhizoctonia Orchid mycorrhiza on Anoectochilus formosanus Hayata |
Source. Vol.(no):Page |
BOTANICAL STUDIES. 48(4): 445-451p. |
Categories |
Orchid Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
|
Host |
Anoectochilus formosanus Hayata |
Organism |
Rhizoctonia solani |
Country |
Taiwan, Asia |
Abstracts |
Rhizoctonia spp. of binucleate R02 and multi-nucleate R04 (Rhizoctonia solani, AG-6) of orchid mycorrhizal fungi (OMF)-collected and isolated from terrestrial orchid roots in Taiwan, including Anoectochilus formosanus Hayata, an orchid native to Taiwan-enhanced the growth of A. formosanus plants to a high degree, both in vitro and ex vitro. OMF (R02 or R04) inoculation used oatmeal agar (OMA) in vitro, in which mycorrhizal seedlings were larger than non-mycorrhizal controls on Hyponex # 3 agar medium. Both Rhizoctonia isolates (R02, R04) inoculated alone or mixed could significantly enhance plant grown ex vitro in terms of plant height, leaf number, root length, and fresh weight. Light and scanning electron microscopy showed that the infection of OMF on A. formosanus was a type of tolypophagy, with hyphal coils and pelotons (mycelial masses) formed in the cortical region of the roots. Mycorrhizal plants showed higher enzyme activities (superoxide dismutase; SOD in leaf, acid and alkaline phosphatases in roots) and markedly higher constituent contents, like ascorbic acid, polyphenols, and flavonoids, which made the mycorrhizal plants a better source of antioxidants, and of polysaccharides and phosphates indicating a more potent medicinal value. Mycorrhizal plants were also susceptible to diseases and mites, so a plastic bag cultivation method was applied and showed itself to be a very effective and labor saving way to grow fungicide- and pesticide-free A. formosanus plants.
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