Record ID No. |
1306 |
Author(s) |
Sujan Singh , 2005 |
Affiliation |
TERI, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi - 110 003, India. |
Title |
Assessment of a metabolically active VAM infection by vital staining methods |
Source. Vol.(no):Page |
Mycorrhiza-News. 17(1): 2-9p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Systematics |
Sub-subjects |
Cultural Studies |
Host |
Plants |
Organism |
Microbes |
Country |
India, Asia |
Abstracts |
The extent of vesicular arbuscular mycorrhizal (VAM) infection in plant roots is usually measured in terms of infected root length as a percentage of total root length, made visible by non-vital stains like trypan blue or chlorozol black. These stains, however, do not differentiate between active and inactive or dead cells of the fungal hyphae. In order to have a clear understanding of the dynamics of VAM infection and the development of fungal hyphae in roots and in soil, the use of vital stains is necessary. This paper contains discussions on: (1) the methods of staining and extraction of enzymes from roots; (2) the extraction of hyphae from VAM roots; (3) the quantification of active VAM infection; (4) the course of VAM infection; (5) the effect of phosphorus on metabolic activity of VAM as determined by enzyme staining; (6) the effect of fungicides on metabolic activity of VAM as determined by enzyme staining; and (7) the effect of interactions with other soil microorganisms on the metabolic activity of VAM as determined by enzyme staining. |