Record ID No. |
1416 |
Author(s) |
Rabie G H., Aboul Nasr M. B., Al Humiany A. , 2004 |
Affiliation |
Botany Department, Faculty of Science, Zagazig Univ., Zagazig, Egypt. |
Title |
Increasing salinity tolerance of cowpea plants by the dual inoculation of Glomus clarum mycorrhiza fungus and the nitrogen-fixer Azospirillum brasilense |
Source. Vol.(no):Page |
African Journal of Mycology and Biotechnology. 12(2): 1-19p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biological Interaction |
Sub-subjects |
Non-nodule forming nitrogen fixers |
Host |
Vigna sinensis, V. unguiculata |
Organism |
Glomus clarum, Azospirillum brasilense |
Country |
Egypt, Africa |
Abstracts |
Pot greenhouse experiments were carried out in an attempt to increase the salinity tolerance of one of the most popular legumes, cowpea (Vigna sinensis [V. unguiculata]), by using dual inoculation of arbuscular mycorrhizal (AM) fungus Glomus clarum and the nitrogen fixing bacterium Azospirillum brasilense. The effect of these beneficial microbes, is single or dual inoculation treatments was assessed in sterilized loamy sand soil at five NaCl levels (0.0-4000 ppm) added to irrigating water. The percentage of mycorrhizal infection, plant height, dry weight, nodule number, protein content, nitrogenase and phosphatase [phosphoric monoester hydrolases] activities, and the nutrient elements N, P, K+, Ca+2, Mg+2 were significantly decreased by increasing the salinity level in non-inoculated plants. Plants inoculated with nitrogen fixing bacteria NFB showed higher nodule numbers, protein content, nitrogen concentration and nitrogenase activities than those of non-inoculated ones at all salinity levels. Mycorrhizal plants showed better improvement in all measurements than that of non-mycorrhizal ones at all salinity levels, especially, in the presence of NFB. The concentration of Na+ was significantly increased in cowpea plants by raising salinity except in shoots of mycorrhizal plants which had K+/Na+ ratios higher than other treatments. Hence, dual inoculation with the AM fungus Glomus clarum and the N-fixer Azospirillum can support the need for both N and P and thus the ability to withstand the excess NaCl tolerance assuring their usefulness for soil recovery in saline area.. |