Record ID No. |
1592 |
Author(s) |
Wilde P., Manal A., Stodden M., Sieverding E., Hildebrandt U., Bothe H. , 2009 |
Affiliation |
Univ Cologne, Inst Bot, Gyrhofstr 15, D-50923 Cologne, GERMANY |
Title |
Biodiversity of arbuscular mycorrhizal fungi in roots and soils of two salt marshes |
Source. Vol.(no):Page |
Environmental Microbiology. 11(6):1548-1561p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Country |
Germany |
Abstracts |
The occurrence of arbuscular mycorrhizal fungi (AMF) was assessed by both morphological and
molecular criteria in two salt marshes: (i) a NaCl site of the island Terschelling, Atlantic Coast, the
Netherlands and (ii) a K2CO3 marsh at Schreyahn, Northern Germany. The overall biodiversity of AMF, based on
sequence analysis, was comparably low in roots at both sites. However, the morphological spore analyses from
soil samples of both sites exhibited a higher AMF biodiversity. Glomus geosporum was the only fungus of the
Glomerales that was detected both as spores in soil samples and in roots of the AMF-colonized salt plants Aster
tripolium and Puccinellia sp. At both saline sites and on all sampling dates (one exception). In roots,
sequences of Glomus intraradices prevailed, but this fungus could not be identified unambiguously from DNA of
soil spores. Likewise, Glomus sp. Uncultured, only deposited as sequence in the database, was widely detected
by DNA sequencing in root samples. All attempts to obtain the corresponding sequences from spores isolated from
soil samples failed consistently. A small sized Archaeospora sp. Was detected, either/or by morphological and
molecular analyses, in roots or soil spores, in dead AMF spores or orobatid mites. The study noted
inconsistencies between morphological characterization and identification by DNA sequencing of the 5.8S
rDNA-ITS2 region or part of the 18S rDNA gene. The distribution of AMF unlikely followed the salt gradient at
both sites, in contrast to the zone formation of plant species. Zygotes of the alga Vaucheria erythrospora
(Xanthophyceae) were retrieved and should not be misidentified with AMF spores. |