Record ID No. |
163 |
Author(s) |
Aono T., Maldonado-Mendoza I. E., Dewbre G. R., Harrison M. J., Saito M. , 2004 |
Affiliation |
National Institute of Agroenvironm Science, Tsukuba, Ibaraki 3058604, Japan |
Title |
Expression of alkaline phosphatase genes in arbuscular mycorrhizas |
Source. Vol.(no):Page |
New Phytologist. 162(2): 525-534 p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry Genetics |
Sub-subjects |
Phosphorus metabolism |
Host |
n.a. |
Organism |
Glomus intraradices, Gigaspora margarita |
Country |
Japan, Asia |
Abstracts |
The authors have identified a partial cDNA clone showing similarity
to the yeast alkaline phosphatase (ALP) gene PHO8 in an EST
library constructed from extraradical hyphae of Glomus
intraradices.Using this clone and 5'-RACE (rapid amplification
of cDNA ends), a full-length cDNA has been cloned for ALP from G.
intraradices. By using RT (reverse transcription)-PCR and
degenerate primers reflecting conserved regions between the
putative amino acid sequences of this cDNA and yeast alkaline
phosphatases, we obtained a full-length ALP cDNA from Gigaspora
margarita. These genes were designated GiALP and GmALP,
respectively.Both GiALP and GmALP were constitutively expressed
in mycorrhizal roots, irrespective of the growth stage,
infection rate, and environmental phosphate concentration. The
levels of GiALP and GmALP transcripts were higher in mycorrhizal
roots than in germinating spores and extraradical hyphae.These
results suggest that arbuscular mycorrhizal ALP is expressed
under symbiotic conditions and that it may have a role in
nutrient exchange with host plants rather than in nutrient
uptake from the rhizosphere.
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