Record ID No. |
1773 |
Author(s) |
Li Y C., Tao W Y., Cheng L. , 2009 |
Affiliation |
Jiangnan Univ, Minist Educ, Key Lab Ind Biotechnol, Sch Biotechnol, Wuxi 214122, Peoples Republic of China |
Title |
Paclitaxel production using co-culture of Taxus suspension cells and paclitaxel-producing endophytic fungi in a co-bioreactor |
Source. Vol.(no):Page |
Applied Microbiology and Biotechnology. 83(2): 233-239p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Country |
China, Asia |
Abstracts |
The co-culture of the suspension cells of Taxus chinensis var. Mairei and its endophytic fungi, Fusarium mairei, in a 20-L co-bioreactor was successfully established for paclitaxel production. The co-bioreactor consists of two-unit tanks (10 L each) with a repairable separate membrane in the center, culturing Taxus suspension cells in one tank and growing fungi in another. By optimizing the co-culture conditions, there was a desirable yield of paclitaxel in Taxus cell cultures. The Taxus cell cultures by co-culture produced 25.63 mg/L of paclitaxel within 15 days; it was equivalent to a productivity of 1.71 mg/L per day and 38-fold higher than that by uncoupled culture (0.68 mg/L within 15 days). The optimum conditions for co-culture in the co-bioreactor were: B5 medium, inoculating fungi when Taxus cells had grown for 5 days in the co-bioreactor, hydrophilic separate membrane in the center of the co-bioreactor, and air flow rate of 1:0.85 v/v/m in fungus cultures. |