Record ID No. |
180 |
Author(s) |
Pierleoni R., Buffalini M., Vallorani L., Guidi C. , Zeppa S., Sacconi C., Pucci P., Amoresano A., Casbarra A. , 2004 |
Affiliation |
University of Urbino, Istitute of Chemical Biology, Via A Saffi 2, I-61029 Urbino, PU, Italy |
Title |
Tuber borchii fruit body: 2-dimensional profile and protein Identification |
Source. Vol.(no):Page |
Photochemistry. 65(7): 813-820p. |
Categories |
Ectomycorrhiza |
Subjects |
Systematics |
Sub-subjects |
Molecular taxonomy |
Host |
n.a. |
Organism |
Truffle, Tuber borchii |
Country |
Italy, Europe |
Abstracts |
The formation of the fruit body represents the final
phase of the ectomycorrhizal fungus T. borchii life cycle. Very
little is known concerning the molecular and biochemical
processes involved in the fructification phase. 2-DE maps of
unripe and ripe ascocarps revealed different protein expression
levels and the comparison of the electropherograms led to the
identification of specific proteins for each developmental
phase. Associating micropreparative 2-DE to microchemical
approaches, such as N-terminal sequencing and 2-D
gel-electrophoresis mass-spectrometry, proteins playing pivotal
roles in truffle physiology were identified.
|