Record ID No. |
1810 |
Author(s) |
Purin, S., Rillig, M.C , 2008 |
Affiliation |
W Virginia University, Div Plant & Soil Sci, 1090 Agr Sci Bldg, POB6108, Morgantown,WV 26506 USA |
Title |
Immuno-cytolocalization of glomalin in the mycelium of the arbuscular mycorrhizal fungus Glomus intraradices |
Source. Vol.(no):Page |
Soil Biology & Biochemistry.40(4):1000-1003p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Organism |
n.a. |
Country |
United States |
Abstracts |
A better understanding of the role of glomalin in arbuscular mycorrhizal fungi
necessitates knowledge about the cellular functions and locations of this putative heat-shock
protein (Hsp). In the present study, we determined the cellular localization of glomalin in
mycelium of Glomus intraradices using immuno-electron microscopy, employing the monoclonal
antibody MAb32B11. We observed that there were more gold beads bound to hyphae and spore walls
than in the cytoplasm. There was also differential binding within the wall layers: L3 and L2
presented more intense labeling than the L1 layer. Our data on wall-binding of glomalin are
strongly suggestive of functions other than cytoplasmic (Hsp-related), and point to a possibility
of mediating interactions with the biotic and abiotic soil environment. (C) 2006 Elsevier Ltd.
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