Record ID No. |
1829 |
Author(s) |
Balestrini R., GomezAriza J., Lanfranco L., Bonfante P , 2008 |
Affiliation |
University of Turin, CNR, Ist Protez Piante, Viale Mattioli 25, I-10125 Turin, ITALY |
Title |
Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells |
Source. Vol.(no):Page |
Molecular Plant - Microbe Interactions.20(9):1055-1062p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Organism |
n.a. |
Country |
ITALY, Europe |
Abstracts |
The establishment of a symbiotic interaction between plant roots and arbuscular
mycorrhizal (AM) fungi requires both partners to undergo significant morphological and
physiological modifications which eventually lead to reciprocal beneficial effects. Extensive
changes in gene expression profiles recently have been described in transcriptomic studies that
have analyzed the whole mycorrhizal root. However, because root colonization by AM fungi involves
different cell types, a cell-specific gene expression pattern is likely to occur. We have applied
the laser microdissection (LMD) technology to investigate expression profiles of both plant and
fungal genes in Lycopersicon esculentum roots colonized by Glomus mosseae. A protocol to harvest
arbuscule-containing cells from paraffin sections of mycorrhizal roots has been developed using a
Leica AS LMD system. RNA of satisfactory quantity and quality has been extracted for molecular
analysis. Transcripts for plant phosphate transporters (LePTs), selected as molecular markers for
a functional symbiosis, have been detected by reverse-transcriptase pollymerase chain reaction
assays and associated to distinct cell types, leading to novel insights into the distribution of
LePT mRNAs. In fact, the transcripts of the five phosphate transporters (PTs) have been detected
contemporaneously in the same arbusculated cell population, unlike from the neighboring
noncolonized cells. In addition, fungal H(+)ATPase (GmHA5) and phosphate transporter (GmosP7)
mRNAs were found exclusively in arbusculated cells. The discovery that five plant and one fungal
PT genes are consistently expressed inside the arbusculated cells provides a new scenario for
plant-fungus nutrient exchanges. |