Record ID No. |
1844 |
Author(s) |
Yamato M., Iwase K , 2008 |
Affiliation |
The General Environmental Technos Co Ltd, Department of Resource & Development, Biol Environm Inst, 8-4 Ujimatafuri, Kyoto 6110021, JAPAN |
Title |
Introduction of asymbiotically propagated seedlings of Cephalanthera falcata (Orchidaceae) into natural habitat and investigation of colonized mycorrhizal fungi |
Source. Vol.(no):Page |
Ecological Research.23(2):329-337p. |
Categories |
Mycorrhiza General |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Organism |
n.a. |
Country |
JAPAN, Asia |
Abstracts |
Asymbiotic seedling propagation and introduction of seedlings into a natural
habitat were achieved for Cephalanthera falcata. For immature seeds collected 65 days after
pollination, high germination rate (av. 50%) was achieved on Hyponex agar medium plates. Root
development occurred in about 10% of the protocorms 5 months after seed sowing. Rooted protocorms
were transferred to a culture bottle containing 100 ml of the Hyponex agar medium and incubated
continually. In about 30% of the transferred individuals, shoot height reached 1.5-2 cm 8 months
after the transfer. After acclimatization in wet vermiculite at 4 degrees C for 6 months, 135
individuals were planted in a natural stand of C. Falcata in mid February 2001. Shoot appearance
rate was 44.4% at the first year and flowering was noted in some plants. At the fifth year,
shoots with an average height of 21.6 cm still appeared in four plants, and flowering was noted
in three of them. Colonization of mycorrhizal fungi was examined in two of them as well as one
wild plant, in which the mycorrhizal fungi were identified to be in Thelephoraceae or
Russulaceae. These fungi are known to form ectomycorrhiza with trees, and thus a tripartnership
symbiosis consisting of C. Falcata, mycorrhizal fungi and trees was suggested. The involvement of
ectomycorrhizal fungi might be the reason for the low survival rate in the field experiment,
because the distribution of ectomycorrhizal fungi relevant to this orchid is assumed to be
uneven. The possibility of introducing artificially propagated orchids into natural habitats was
discussed. |