Record ID No. |
188 |
Author(s) |
Cardoso I. M., Boddington C. L., Janssen B. H., Oenema O., Kuyper T. W. , 2004 |
Affiliation |
Wageningen University, Department of Soil Quality, POB 8005, NL-6700 EC Wageningen, Netherlands |
Title |
Double pot and double compartment: Integrating two approaches to study nutrient uptake by arbuscular mycorrhizal fungi |
Source. Vol.(no):Page |
Plant and Soil. 260(1-2): 301-310p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Soil plant relations |
Sub-subjects |
Nutrition |
Host |
Maize |
Organism |
Glomus spp. |
Country |
The Netherlands, Europe |
Abstracts |
The double compartment technique has been commonly used
in studies on nutrient uptake by mycorrhizas whereas the double
pot technique has been used to assess the nutritional stress of
plants grown in different soils. A combination of the double pot
and the double compartment technique was used as a tool to
understand the processes involving mycorrhiza and plant
nutrition. Maize (Zen mays) and three species of the arbuscular
mycorrhizal fungi in the genus Glomus were used to study
phosphorus (P) uptake with and without mycorrhiza from the A and
B horizons of an Oxisol. The plants were supplied from the lower
pot with a nutrient solution without P. The upper pot had a
double compartment with either a fine and coarse mesh screen to
control the volume of soil explored by the roots, and thereby,
limit the amount of soil P accessible to plants from the test
soil. There were significant effects of time for plants grown in
both soil horizons, and of mycorrhizas for plants grown in the A
soil horizon. No effect of mesh size was observed. There were
significant effects of horizon and mycorrhiza, but not of mesh
size, on the dry weight and P contents of shoots and roots. The
P concentration (P g kg(-1) of plant) for shoots and roots was
similar in the A and B soil horizons. The results showed that
the double pot - double compartment system was suitable for the
experimental objectives. No differences in plant growth were
observed when root growth was not limited vs root growth limited
to the inner compartment because the non-mycorrhizal plants did
not take up P. Consequently, responsiveness of the maize
cultivar was wholly dependent on mycorrhiza for P uptake under
these experimental conditions.
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