Record ID No. |
1979 |
Author(s) |
Zadworny M., Tuszynska S., Samardakiewicz S., Werner A , 2007 |
Affiliation |
Polish Acad Sci, Inst Dendrol, Lab Root Pathol, PL-62035 Kornik, POLAND |
Title |
Effects of mutual interaction of Laccaria laccata with Trichoderma harzianum and T-virens on the morphology of microtubules and mitochondria |
Source. Vol.(no):Page |
Protoplasma.232(1-2):45-53p. |
Categories |
Ectomycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Organism |
n.a. |
Country |
POLAND,Europe |
Abstracts |
Organelles are known to respond to challenges caused by many stress factors. The
morphology of the microtubular cytoskeleton and mitochondria during mutual interaction in
coculture of Laccaria laccata with Trichoderma harzianum and T. Virens were examined. Hyphae from
the interaction region were sampled between 4 and 12 days of growth. Microtubules were labelled
with a specific antibody and mitochondria with 3,3'-dihexyloxacarbocyanine iodide, and the
organelles were examined microscopically. The morphology of microtubules and mitochondria were
similar in all three fungi. Microtubules were arranged in long arrays parallel to the hyphal axis
and mitochondria formed an interconnected network. In hyphae growing within the interaction zone,
microtubules became wavy and eventually fragmented or depolymerised, and mitochondria also became
fragmented. The effects were time-dependent. In general, the organelles of all three fungi were
affected during the interaction, but L. Laccata was affected the least and to the same extent by
each of the saprotrophic fungi. The saprotrophic fungi were affected by L. Laccata to a similar
extent at 4 and 8 days of interaction. Our results suggest that the studied fungi
antagonistically affect each other at the cellular level, although the mechanisms involved remain
to be elucidated. |