Record ID No. |
1996 |
Author(s) |
Jahromi F., Aroca R., Porcel R., RuizLozano J.M , 2008 |
Affiliation |
CSIC, Estac Expt Zaidin, Dept Microbiol Suelo & Sist Simbiot, Prof Albareda 1, E-18008 Granada, SPAIN |
Title |
Influence of salinity on the In vitro development of Glomus intraradices and on the In vivo physiological and molecular responses of mycorrhizal lettuce plants |
Source. Vol.(no):Page |
Microbial Ecology.55(1):45-53p. |
Categories |
Mycorrhiza General |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Organism |
n.a. |
Country |
SPAIN, Europe |
Abstracts |
Increased salinization of arable land is expected to have devastating global
effects in the coming years. Arbuscular mycorrhizal fungi (AMF) have been shown to improve plant
tolerance to abiotic environmental factors such as salinity, but they can be themselves
negatively affected by salinity. In this study, the first in vitro experiment analyzed the
effects of 0, 50, or 100 mM NaCl on the development and sporulation of Glomus intraradices. In
the second experiment, the effects of mycorrhization on the expression of key plant genes
expected to be affected by salinity was evaluated. Results showed that the assayed isolate G.
Intraradices DAOM 197198 can be regarded as a moderately salt-tolerant AMF because it did not
significantly decrease hyphal development or formation of branching absorbing structures at 50 mM
NaCl. Results also showed that plants colonized by G. Intraradices grew more than nonmycorrhizal
plants. This effect was concomitant with a higher relative water content in AM plants, lower
proline content, and expression of Lsp5cs gene (mainly at 50 mM NaCl), lower expression of the
stress marker gene Lslea gene, and lower content of abscisic acid in roots of mycorrhizal plants
as compared to nonmycorrhizal plants, which suggest that the AM fungus decreased salt stress
injury. In addition, under salinity, AM symbiosis enhanced the expression of LsPIP1. Such
enhanced gene expression could contribute to regulating root water permeability to better
tolerate the osmotic stress generated by salinity.
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