Record ID No. |
2029 |
Author(s) |
Chen C.Y., Gao M.Q., Liu J.Y., Zhu H.Y , 2007 |
Affiliation |
University of Kentucky, Department of Plant & Soil Science, Lexington,KY 40546 USA |
Title |
Fungal symbiosis in rice requires an ortholog of a legume common symbiosis gene encoding a Ca2+/calmodulin-dependent protein kinase(1[OA]) |
Source. Vol.(no):Page |
Plant Physiology.145(4):1619-1628p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Organism |
n.a. |
Country |
United States |
Abstracts |
In natural ecosystems, many plants are able to establish mutually beneficial
symbioses with microorganisms. Of critical importance to sustainable agriculture are the
symbioses formed between more than 80% of terrestrial plants and arbuscular mycorrhizal (AM)
fungi and between legumes and nitrogen-fixing rhizobial bacteria. Interestingly, the two
symbioses share overlapping signaling pathways in legumes, suggesting that the evolutionarily
recent root nodule symbiosis may have acquired functions from the ancient AM symbiosis. The
Medicago truncatula DMI3 (DOESN'T MAKE INFECTIONS3) gene (MtDMI3) and its orthologs in legumes
are required for both bacterial and fungal symbioses. MtDMI3 encodes a Ca2+/calmodulin-dependent
protein kinase (CCaMK) essential for the transduction of the Ca2+ signal induced by the
perception of Nod factors. Putative orthologs of MtDMI3 are also present in non-legumes, but
their function in AM symbiosis has not been demonstrated in any non-legume species. Here, we
combine reverse genetic approaches and a cross-species complementation test to characterize the
function of the rice (Oryza sativa) ortholog of MtDMI3, namely, OsDMI3, in AM symbiosis. We
demonstrate that OsDMI3 is not only required for AM symbiosis in rice but also is able to
complement a M. Truncatula dmi3 mutant, indicating an equivalent role of MtDMI3 orthologs in
non-legumes. |