Record ID No. |
203 |
Author(s) |
Liu A., Wang B., Hamel C. , 2004 |
Affiliation |
McGill University, Department of Natural Resource Sciences, Macdonald Campus,21111 Lakeshore Rd, Ste Anne De Bellevue, PQ H9X 3V9, Canada, Email: liu_aiguo@hotmail.com |
Title |
Arbuscular mycorrhiza colonization and development at suboptimal root zone temperature |
Source. Vol.(no):Page |
Mycorrhiza. 14(2): 93-101 p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Soil plant relations |
Sub-subjects |
Soil temperature |
Host |
Sorghum bicolor, Daucus carota |
Organism |
Glomus intraradices |
Country |
Canada, N. America |
Abstracts |
Temperature has a strong influence on the activity of living organisms. This study, involving two indoor experiments, evaluated the effects of root zone temperature (10, 15 and 23degreesC) on the formation and development of arbuscular mycorrhizae (AM). In the first trial, greenhouse-grown sorghum [Sorghum bicolor (L.) Moench] was either colonized by Glomus intraradices Schenck & Smith or left non-mycorrhizal. Root length, root and shoot weight and root colonization were measured after 5, 10 and 15 weeks of plant growth. Although suboptimal root zone temperatures reduced growth in both mycorrhizal and non-mycorrhizal plants, mycorrhizal plants were larger than non-mycorrhizal plants after 15 weeks at 15 and 23degreesC. At suboptimal root zone temperatures, mycorrhizal inoculation sometimes slightly reduced root development. AM colonization was more affected than root growth at suboptimal root zone temperatures. Colonization was markedly reduced at 15degreesC compared with 23degreesC, and almost completely inhibited at 10degreesC. The second experiment was conducted in vitro using transformed carrot (Daucus carota L.) roots supporting G. intraradices. Mycelium length and spore number were measured weekly for 15 weeks. Spore metabolic activity (iodonitrotetrazolium reduction), root length and percentage root colonization were measured after 15 weeks. G. intraradices sporulation was reduced at temperatures below 23degreesC, while spore metabolic activity was significantly reduced only at 10degreesC. Root length and in particular percentage colonization were decreased at suboptimal temperatures. A negative interaction between AM hyphal growth and root growth resulting in reduced probability of contact at suboptimal root zone temperatures is proposed to explain the greater reduction observed in root colonization than in root and hyphal growth. |