Record ID No. |
2041 |
Author(s) |
Olsson, PA; Hammer, EC; Wallander, H; Pallon, J. , 2008 |
Affiliation |
Olsson PA, Lund Univ, Dept Plant Ecol & Systemat, Ecol Bldg, SE-22362 Lund, SWEDEN |
Title |
Phosphorus availability influences elemental uptake in the mycorrhizal fungus Glomus intraradices, as revealed by particle-induced X-ray emission analysis |
Source. Vol.(no):Page |
Applied and Environmental Microbiology, 74(13): 4144-4148 |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Country |
Sweden, Europe |
Abstracts |
We investigated element accumulation in the arbuscular mycorrhizal fungus Glomus intraradices. Fungal spores and mycelia growing in monoxenic cultures were analyzed. The elemental composition was quantified using particle-induced X-ray emission (PIXE) in combination with scanning transmission ion microscopy. In the spores, Ca and Fe were associated mainly with the spore wall, while P and K showed patchy distributions and their concentrations were correlated. Excess of P in the hyphal growth medium increased the P and Si concentrations in spores and increased the K/Ca ratio in spores. Increased P availability decreased the concentration of Zn and Mn in spores. We concluded that the availability of P influences the uptake and accumulation of several elements in spores. It is demonstrated that PIXE analysis is a powerful tool for quantitative analysis of elemental accumulation in fungal mycelia. |