Record ID No. |
224 |
Author(s) |
Wirsel S.G.R. , 2004 |
Affiliation |
Univ Halle Wittenberg, Inst Pflanzenzuchtung & Pflanzenschutz, Ludwig Wucherer Str 2, D-06099 Halle Saale, Germany |
Title |
Homogenous stands of a wetland grass harbour diverse |
Source. Vol.(no):Page |
FEMS MICROBIOLOGY ECOLOGY. 48(2): 129-138p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Genetics |
Host |
n.a |
Organism |
Glomus Scutellospora, Acaulospora, Glomus mosseae, Glomus fasciculatum |
Country |
Germany, Europe |
Abstracts |
A molecular approach was applied to investigate the
colonisation of arbuscular mycorrhizal fungi (AMF) on the
wetland grass Phragmites australis. A PCR assay targeting the
traditional families of the Glomeromycota yielded products that
were used to construct libraries of 18S rDNA. Five hundred and
forty six clones were typed by restriction analysis and 76
representatives were sequenced. The majority corresponded to a
wide range of taxa within Glomus group A, a few belonged to the
"Diversisporaceae" and none to the genera Scutellospora or
Acaulospora. Among these sequences, some were very similar to
those reported earlier, e.g. Glomus mosseae and G. fasciculatum,
other pointed to various new taxa. Although this wetland habitat
harboured just one single plant species, phylogenetic analysis
exhibited 21 AMF phylotypes, which is in the same range as
reported for other natural ecosystems composed of more diverse
host communities. Diversity indices supported the perception
that the AM F mycoflora associated with this natural grass
"monoculture" is not depauperate as it had been described for
grasses of crop monocultures. Soil conditions determined the
mycorrhizal state of the host, since AMF were not detected at
the lakeward front of the reed belt, which is permanently
waterlogged
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