Record ID No. |
2401 |
Author(s) |
Recorbet G; Valot B; Robert F; Gianinazzi-Pearson V; Dumas-Gaudot E. , 2010 |
Affiliation |
UMR 1088 INRA/CNRS 5184/UB Plante-Microbe-Environnement, INRA-CMSE, BP86510, 21065 Dijon Cedex, France. recorbet@dijon.inra.fr |
Title |
Identification of in planta-expressed arbuscular mycorrhizal fungal proteins upon comparison of the root proteomes of Medicago truncatula colonised with two Glomus species |
Source. Vol.(no):Page |
Fungal Genet Biol 47(7): 608-618p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Host |
Medicago truncatula |
Organism |
Glomus mosseae, G. intraradices |
Country |
France, Europe |
Abstracts |
In the absence of sequenced genomes for arbuscular mycorrhizal (AM) fungi, their obligatory biotrophy makes their intra-radical biology especially recalcitrant to functional analyses. Because tandem mass spectrometry-based proteomics enables fungal gene product identifications in phyla lacking genomic information, we have compared as a way to enlarge the coverage of in planta expressed-mycorrhiza-related proteins, the root proteome responses of Medicago truncatula upon colonisation with two AM fungi, Glomus mosseae and G. intraradices, using two-dimensional electrophoresis. In contrast to phosphate fertilization, mycorrhization led to specific changes in the abundance of 99 spots, including 42 overlapping modifications between G. mosseae- and G. intraradices-colonised roots. The 32 confident identifications that could be retrieved following tandem mass spectrometry encompassed 21 fungal proteins whose homology-inferred functions were found to complement the working models so far proposed for the intra-radical functioning of AM fungi with regard to carbon utilization, energy generation, redox homeostasis and protein turnover-related processes. |