Record ID No. |
2446 |
Author(s) |
Zhu LiHua., Cheng Fang., Qin Li., Ye JianRen. , 2010 |
Affiliation |
Jiangsu Key Laboratory for Prevention and Management of Invasasion Species, College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China. lhzhu@njfu.com.cn; jrye@njfu.edu.cn |
Title |
Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii |
Source. Vol.(no):Page |
Journal of Zhejiang Forestry College. 27(3):398-403p. |
Categories |
Ectomycorrhiza |
Subjects |
Soil plant relations |
Sub-subjects |
Tissue Culture |
Host |
Pinus elliottii |
Organism |
Pisolithus tinctorius |
Country |
China, Asia |
Abstracts |
The condition for axillary bud induction of Pinus elliottii has been optimized, and mycorrhizas on the rooted explants has been tested in vitro to improve the efficiency of micropropagation. Axillary bud induction was found to be influenced by the basal medium as well as the age of the seedling from which the explant was taken. Modified Gresshoff and Doy's (GD) medium proved to be superior to others, and the right stage of seedling was 18-25 d. The addition of activated charcoal (0.5 mg.L-1) stimulated shoot elongation. Rooting occurred at about 80% on 1/2GD medium supplemented with 0.05 mg.L-1 NAA and 1.0 mg.L-1 IBA. Mycorrhizas were initiated for plantlets with Pisolithus tinctorius on an agar medium under fully controlled conditions. Mycorrhizas improved the transfer of the plantlets to ex vitro conditions. After 3 years in the field, the plantlets reached an average of 80.3 cm in height. Their shoot growth appearance was normal. |