Record ID No. |
246 |
Author(s) |
Barbieri E., Gioacchini A.M., Zambonelli A., Bertini L., Stocchi V. , 2005 |
Affiliation |
Università degli Studi di Urbino, Ist Ric Attivita Motoria, Carlo Bo,Via Sasso 75-G, I-61029 Urbino, Italy |
Title |
Determination of microbial volatile organic compounds from Staphylococcus pasteuri against Tuber borchii using solid-phase microextraction and gas chromatography/ion trap mass spectrometry |
Source. Vol.(no):Page |
Rapid communications in mass spectrometry. 19(22): 3411-3415 p. |
Categories |
Ectomycorrhiza |
Subjects |
Methodology |
Sub-subjects |
Soil microfauna |
Host |
Trees |
Organism |
Staphylococcus pasteuri, Tuber borchii |
Country |
Italy, Europe |
Abstracts |
The mycelium of Tuber borchii Vittad., a commercial truffle species, is used as a model system for in vitro ectomycorrhizal synthesis, infected seedling production and biotechnological applications. Our fungal cultures were accidentally contaminated with a Staphylococcus pasteuri strain, showing a strong antifungal activity against T. borchii mycelium. In order to identify the antifungal volatile agents produced by S. pasteuri, solid-phase microextraction (SPME) with gas chromatography and mass spectrometry (GC/MS) was used. Using this method 65 microbial volatile organic compounds (MVOCs), synthesized by this bacterium in either single or in fungal-bacterial dual culture, were identified. SPME combined with GC/MS may be a useful method for the determination of MVOCs involved in the antifungal activity. These results showed that bacteria with unusual biological activities could be a major problem during large-scale production of inoculum for truffle-infected seedling.
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