Record ID No. |
2525 |
Author(s) |
Wang JingJing., Sun ShuBin., Xu GuoHua. , 2010 |
Affiliation |
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. ghxu@njau.edu.cn |
Title |
An in vitro dual culturing system established with tomato hairy roots and arbuscular mycorrhizal fungi |
Source. Vol.(no):Page |
Mycosystema. 29(1):68-74p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Genetics |
Sub-subjects |
|
Host |
Lycopersicon esculentum |
Organism |
Agrobacterium rhizogenes, Glomus intraradices |
Country |
China., East Asia |
Abstracts |
Agrobacterium rhizogenes A4 was used to induce Lycopersicon esculentum Micro-Tom hairy roots. An in vitro dual culturing system was established with Ri T-DNA transformed Micro-Tom roots and Glomus intraradices. Multilevel-branched hyphae were observed in about 14 days, and some hyphal top became expanded, and gradually produced some newborn spores in about 17-21 days of colonization. About 600-800 mature spores per dish were obtained in 3 months of dual culture. The harvested spores were capable of germination and subsequently colonizing the host hairy roots immediately. The successful in vitro dual culture of tomato hairy roots and mycorrhizal fungi provided the ideal experimental system for conducting the research on physiological and molecular mechanism of tomato mycorrhiza. |