Record ID No. |
282 |
Author(s) |
Sykorova Z., Rydlova J., Vosatka M. , 2003 |
Affiliation |
Academy of Science of the Czech Republic, Institute of Botony, CZ-25243 Pruhonice, Czech Republic |
Title |
Establishment of mycorrhizal symbiosis in Gentiana verna |
Source. Vol.(no):Page |
Folia Geobotanica. 38(2): 177-189p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Physiology |
Sub-subjects |
|
Host |
Gentiana verna |
Organism |
AMF |
Country |
Czech Republic, Europe |
Abstracts |
The last lowland locality of Gentiana verna in the Czech Republic is a calcareous grassland near Rovna at Strakonice in South Bohemia. This locality was the subject of a recovery programme that included support of the remaining population by micropropagation. The plants were inoculated with arbuscular mycorrhizal fungi (AMF) after their transfer to ex vitro and the effect of AMF on their establishment and survival was studied. Although the conventional method of inoculation of G. Verna using spores or colonized root segments as an inoculum source resulted in no or negligible root colonization, the transplantation of gentians to the locality Rovna was successful and the plants became colonized with AMF very rapidly in the field. Successful mycorrhization of gentians under experimental conditions occurred only via the extraradical mycelial network established by neighbouring mycorrhizal plant species (nurse plant effect). Different nurse plant species formed different morphological types of mycorrhiza when inoculated with the same fungal isolate. Gentians always had the Paris type of root colonization with intracellular hyphal loops and swellings. Intercellular hyphae, arbuscules and vesicles were not observed. No evidence for a positive growth response was found in G. Verna. |