Record ID No. |
2838 |
Author(s) |
Liu Hui, Wu XiaoQin, Chen Dan , 2010 |
Affiliation |
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China. liuhui845@sohu.com; xqwu@njfu.edu.cn |
Title |
Ability of dissolving insoluble phosphate by four ectomycorrhizal fungi |
Source. Vol.(no):Page |
Acta Botanica Boreali-Occidentalia Sinica. 30(1): 143-149p. |
Categories |
Ectomycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Phosphorus metabolism |
Host |
EcM |
Organism |
Xerocomus chrysenteron, Boletus edulis, Rhizopogen luteous, Lactarius insulsus. |
Country |
China, Asia |
Abstracts |
To study the ectomycorrhizal fungi on the dissolution of insoluble phosphate and its possible influencing factors, we used four strains of ectomycorrhizal fungi, Xerocomus chrysenteron, Boletus edulis, Rhizopogen luteous and Lactarius insulsus, to compare their phosphate-dissolving ability in vitro. The results showed that all the four ectomycorrhizal fungi could grow on the solid plate of NBRIP with Ca3 (PO4)2 as sole phosphate source, but having different growth rates. It was found that there were significantly different in dissolving these phosphates of Ca3 (PO4)2, Ca10 (PO4)6 (OH)2, FePO4.4H2O and AlPO4 after culturing 14 days. The amounts of soluble P in the broth of Xerocomus chrysenteron were significantly higher than that of control and other ectomycorrhizal fungi. The titrable acidity of the broth (2.30-46.00 mmol.L-1) was found to be significantly positively correlated to the amounts of soluble P (r=0.991**). Nevertheless there was a negative correlation between the final pH values of the broth and the amounts of soluble P (r=-0.939**). The kinds and concentrations of organic acid were determined by HPLC, all the four strains of ectomycorrhizal fungi were able to excrete Acetic, Citric and Famaric acid. However, significant differences existed between the total of organic acid and the phosphate-dissolving capacity (r=0.998*). The main factors influencing dissolving phosphate on Ca3 (PO4)2 included the titrable acidity, pH value and organic acid excreted by ectomycorrhizal fungi. Therefore, Xerocomus chrysenteron, which had larger phosphate-dissolving ability, is the most promising fungus for afforestation at P-deficient areas in China. |