Record ID No. |
3020 |
Author(s) |
Kedi B., Abadie J., Sei J., Quiquampoix H., Staunton S. , 2010 |
Affiliation |
UMR Eco&Sol, INRA-IRD-Supagro, 2 place Viala 34060 Montpellier cedex 02, France. email: staunton@supagro.inra.fr |
Title |
Interaction of enzymes with soil colloids: adsorption and ectomycorrhizal phosphatase activity on tropical soils |
Source. Vol.(no):Page |
Proceedings of the 19th World Congress of Soil Science, 25-28p. |
Categories |
Ectomycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Phosphorus metabolism |
Host |
N.A |
Organism |
Hebeloma cylindrosporum, Suillus |
Country |
France, Europe. |
Abstracts |
Ectomycorrhizal fungi phosphatases may play a significant role in phosphorus nutrition by the solubilisation of soil organic phosphorus, and this may be of particular importance for highly weathered tropical soils. The expression of phosphatase catalytical activity is highly pH dependent and may be modified to various degrees when in contact with soil colloids. This modification is expected to depend on the nature of the organo-mineral surfaces. The adsorption and modification of acid phosphatase activity, of both intra and extracellular enzymes obtained from three ectomycorrhizal fungi strains in contact with three tropical soils, were studied. The effect of physical fractionation (clay-sized fraction and sieved soil) and chemical cleaning (treatment with H2O2 or dithionite-citrate-bicarbonate to remove organic matter or iron oxides) of the organo-mineral surfaces has been studied. Measurements of catalytic activity were made in the range of pH 3-7. The extent of adsorption and the resulting change in catalytic activity varied between fungal strains, and even for a given strain between extra and intracellular enzymes. The difference between soils was rather small, and neither physical nor chemical treatments had marked effects on the interaction with enzymes.
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