Record ID No. |
3035 |
Author(s) |
Chandran R P., Potty V P. , 2011 |
Affiliation |
Department of Biotechnology, K.V.M. College of Engineering and Information Technology, Kokkothamangalam P. O., Cherthala - 688583. Kerala State, India. e-mail: drpratapchandran@yahoo.co.in |
Title |
Initiation of hairy roots from Canavalia sp. using Agrobacterium rhizogenes 15834 for the co-cultivation of Arbuscular mycorrhizal fungi, Glomus microcarpum |
Source. Vol.(no):Page |
International Journal of Agricultural Technology, 7(2): 235-245p |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Systematics |
Sub-subjects |
Cultural Studies |
Host |
Canavalia.Sp., Ipomoea batatas |
Organism |
Glomus microcarpum, Rhizobium rhizogenes |
Country |
India, Asia |
Abstracts |
Arbuscular mycorrhizal (AM) fungi have symbiotic relationship with plants and which mainly helps the plants for the uptake of phosphorus, other micro nutrients and are fundamental for soil fertility and plant nutrition. The obligate biotrophic nature of AM fungi makes it difficult to grow them in synthetic medium and this prevents large scale inoculum production. In the present investigation, we were successful in raising hairy roots from Canavalia sp. using Agrobacterium rhizogenes ATCC 15834 and successfully co-cultivated AM fungi Glomus microcarpum in hairy roots. The hairy root obtained was confirmed for the presence of rol B genes of Agrobacterium rhizogenes. Different stages of AM fungal colonization were also observed and 60% of mycorrhizal colonization was observed on the 20th day of co-cultivation in petri dish. Mycorrhized Canavalia hairy roots were tested for its potential to use as an mycorrhizal inoculum to infect Ipomoea batatas roots were tested and its showed 76% colonization.
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