Record ID No. |
3168 |
Author(s) |
Chandran R P., Potty V P. , 2010. |
Affiliation |
Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala State, India, Email: drpratapchandran@yahoo.co.in. |
Title |
In vitro co-culture of VAM fungi Glomus microcarpum in Ri T-DNA transformed hairy roots of Vigna vexillata |
Source. Vol.(no):Page |
International Journal of Agriculture Environment & Biotechnology. 3(3): 333-338p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Systematics |
Sub-subjects |
Cultural Studies |
Host |
Vigna vexillata |
Organism |
Glomus microcarpum |
Country |
India, Asia. |
Abstracts |
Vesicular arbuscular mycorrhizal (VAM) fungal association is found in 80% of the plant species and most plant families except Cruciferae, Chenopodiaceae, Caryophyllaceae and Cyperaceae. The obligate biotrophic nature of VAM fungi, limits in vitro culture and large-scale production of mycorrhizal inoculum. This reduced their potential for use as inocula in agricultural and horticultural practices. The establishment of in vitro root organ culture has great potential in understanding the arbuscular mycorrhizal symbiosis and monoxenic inoculum production. In the present investigation hairy roots were initiated from Vigna vexillata (L.) A. Rich. through the mediation of Agrobacterium rhizogenes ATCC 15834. This served as an in vitro system for the co-cultivation of vesicular arbuscular mycorrhizal fungi (Glomus microcarpum), which were grown in modified Murashige and Skoog medium. Cotyledons and hypocotyls segments of V. vexillata were used as explants and hairy roots emerged form the bacterial infected sites. Plant phenolic compound acetosyringone was also used as an inducer molecule to potentiate hairy root infection. Eighty percent mycorrhizal colonization was observed after 20 days of co-cultivation. Various stages of mycorrhizal infection, pre-infection, penetration, vesicles and arbuscule formation were observed and this confirmed the mycorrhizal colonization. VAM fungal cultivation in transgenic hairy roots is a powerful tool to study its biology and this technique can be used for the mass production of monoxenic inocula of VAM fungi. |