Record ID No. |
3200 |
Author(s) |
Devikrishna S., Kumutha K., Santhanakrishnan P., Priya L S. , 2010. |
Affiliation |
Hind Agriculture and Research Institute. |
Title |
Evaluation of AM spores and mycorrhizal root fragment as fungal inoculum for establishing colonization on the Ri-T DNA transformed hairy roots |
Source. Vol.(no):Page |
International Journal of Plant Protection. 3(1): 11-16p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Systematics |
Sub-subjects |
Cultural Studies |
Host |
Solanum lycopersicum(Tomato), Vigna unguiculata(Cow pea) |
Organism |
Glomus caledonium, G. intraradices, G. mosseae |
Country |
India, Asia. |
Abstracts |
Arbuscular mycorrhizal fungi are ubiquitous and form symbiosis with roots of a majority of higher plants for establishing in vitro cultures. Hairy roots can serve as potential host under root organ culturing. Lab experiments were conducted to evaluate the potential of AM spores as well as AM roots to establish colonization in hairy roots. Glomus mosseae, Glomus intraradices and Glomus caledonium in spores as well as colonized roots pieces were used to develop colonization in Ri-TDNA transformed hairy roots of cowpea and tomato using Modified White's Medium (MWM) and Minimal medium (MM). Inoculated plants were kept under dark at 25 degrees C for about 3-4 weeks. When surface sterilized spores were used as inoculum for the establishment of AM colonization in Ri T-DNA transformed hairy roots, G. intraradices produced in M medium the maximum colonization in cowpea and tomato hairy roots, respectively. When mycorrhizal root fragments were used as inoculum, Glomus mosseae produced more colonization in M medium (30.3%) than others in hairy roots of both cowpea and tomato. While comparing the efficiency of two sources two to three fold increase in root colonization was observed in cowpea and tomato hairy roots with the inoculation of AM spores rather than AM root pieces. |