Record ID No. |
3205 |
Author(s) |
Devikrishna S., Kumutha K., Santhanakrishnan P., Priya L S. , 2010. |
Affiliation |
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, COIMBATORE (T.N.) INDIA |
Title |
Influence of surface sterilization and cold treatment on germination of AM spores |
Source. Vol.(no):Page |
Asian Journal of Bio Science. 5(1): 58-62p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
General |
Sub-subjects |
Miscellaneous |
Host |
Crops |
Organism |
Glomus caledonium, G. intraradices, G. mosseae |
Country |
India, Asia. |
Abstracts |
Vesicular arbuscular mycorrhizal (VAM) fungi are a unique group of ubiquitous soil microorganism known to form symbiotic association with roots of economically important crop plants. AM propagules such as isolated spores and sheared mycorrhizal roots are virtually able to initiate AM symbiosis and establish the pre-symbiotic phase with the transformed root. The spores were sterilized with 96% ethyl alcohol and treated for 30, 60 and 90 seconds. Surface sterilization of AM spore viz., Glomus mosseae, G. intrardices and G. caledonium with 96% ethyl alcohol for 60 sec exhibited higher germination percentage without affecting the viability of spores. Further cold treatment of spores at 4 degrees C for 30 days improved the spore germination by 90 and 56.5% in Glomus intraradices and Glomus caledonium, respectively, whereas Glomus mosseae, recorded the maximum germination with 20 days of cold treatment. Though germination was observed higher with 30 days and cold treatment, stratification at 4 degrees C for 16-20 days resulted in better spore germination. |