Record ID No. |
3672 |
Author(s) |
Hossain MS, Liao J, James EK, Sato S, Tabata S, Jurkiewicz A, Madsen LH, Stougaard J, Ross L, Szczyglowski K. , 2012 |
Affiliation |
Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, Canada N5V 4T3. |
Title |
Lotus japonicus ARPC1 is required for rhizobial infection |
Source. Vol.(no):Page |
Plant Physiology, 160 (2): 917-928p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biological Interaction |
Sub-subjects |
Nodule forming nitrogen fixers |
Host |
Lotus japonicus |
Organism |
Rhizobium |
Country |
Canada, N. America |
Abstracts |
Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.
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