Record ID No. |
3976 |
Author(s) |
Wang S, Wang F, Diao X, He L , 2014 |
Affiliation |
Department of Environmental Engineering and Science, Beijing University of Chemical Technology, Beijing 100029, China, email: (Diao X)diaoxiaojun2000@126.com |
Title |
Effects of elevated O3 on microbes in the rhizosphere of mycorrhizal snap bean with different O3 sensitivity |
Source. Vol.(no):Page |
Canadian Journal of Microbiology, 60(2): 93-103p. |
Categories |
Mycorrhiza General |
Subjects |
Genetics |
Sub-subjects |
Climate change |
Host |
Phaseolus vulgaris |
Organism |
Arbuscular Mycorrhiza (AM) |
Country |
China, East Asia |
Abstracts |
Elevated ozone (O3) generally affects microbial biomass and community structure in rhizosphere, but these effects are unclear in mycorrhizal plants because arbuscular mycorrhizal (AM) fungi often benefit microbial growth in the rhizosphere. Here, we investigate the effects of elevated O3 on microbial biomass and community structure in the rhizosphere of mycorrhizal snap bean (Phaseolus vulgaris L.) with different O3 sensitivity (R123: O3-tolerant plant; S156: O3-sensitive plant) based on the phospholipid fatty acids (PLFAs) method. Compared with ambient O3, elevated O3 significantly decreased mycorrhizal colonization rates in the 2 genotypes, especially in S156 plants. The wet masses of shoot and root were decreased by elevated O3 in the 2 genotypes independent of AM inoculation, but they were higher in the mycorrhizal plant than in the nonmycorrhizal plant independent of O3 concentration. Elevated O3 significantly decreased the relative proportion of specific fungal PLFAs in the nonmycorrhizal plant, but this effect disappeared in the mycorrhizal plant. The relative proportions of specific PLFAs of other microbial groups (Gram-positive, Gram-negative, and actinomycete) in the rhizosphere and all specific PLFAs in the hyphosphere were not affected by elevated O3 independent of AM inoculation. In the rhizosphere of the 2 genotypes, microbial community structure was changed by AM inoculation and elevated O3 as well as by their interaction; in the hyphosphere, however, microbial community structure was changed by elevated O3 only in R123 plants. It is concluded that AM inoculation can offset negative effect of elevated O3 on fungal biomass but seems to enhance shift of microbial community structure in rhizosphere under elevated O3. |