Record ID No. |
3982 |
Author(s) |
Periyasamy Panneerselvama*, Boya Saritha, Sukhada Mohandas, Kaushal Kishore Upreti, Seenivasan Poovarasan, Vijay Virupakshayya Sulladmath & Rangarajan Venugopalan , 2013 |
Affiliation |
Division of Soil Science and Agriculture Chemistry, Soil Microbiology Lab, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, India. Email: panneerccri@yahoo.co.in |
Title |
Effect of mycorrhiza-associated bacteria on enhancing colonization and sporulation of Glomus mosseae and growth promotion in sapota (Manilkara achras (Mill) Forsberg) seedlings |
Source. Vol.(no):Page |
Biological Agriculture & Horticulture, 29(2): 118-131p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biological Interaction |
Sub-subjects |
Mycorrhiza helper bacteria |
Host |
Manilkara achras (Mill) Forsberg (Sapota) |
Organism |
Glomus mosseae, Brevibacillus parabrevis, B. choshinensis, Pseudomonas putida, Providencia rettgeri |
Country |
India, South Asia |
Abstracts |
Satisfactory establishment of sapota is one of the major challenges in India due to poor seedling growth in the nursery. A study was undertaken on co-inoculation of sapota (Manilkara achras (Mill) Forsberg) seedlings with mycorrhiza fungus (Glomus mosseae) and mycorrhiza-associated bacteria (MAB) to establish healthy sapota seedlings under nursery conditions. Based on the survey from different sapota cropping systems in India, four bacteria were isolated from G. mosseae spores and identified as Brevibacillus parabrevis (HM590702), Pseudomonas putida (HM590707), B. choshinensis (HM590701) and Providencia rettgeri (HM590704) by polymerase chain reaction amplification of 16S rRNA. These four bacterial isolates were chosen because of their growth-promoting attributes; phytohormones, siderophore and hydrogen cyanide production; antifungal and enzyme activity; and phosphorus and zinc solubilization, and were evaluated with G. mosseae for growth promotion and mycorrhizal development in sapota seedlings. Among these isolates, application of P. putida with G. mosseae significantly increased seedling height, fresh and dry shoot and root weight, total biomass, total leaf area and mycorrhizal colonization compared with other bacterial isolates. The present study findings clearly demonstrated that healthy sapota seedlings can be established by co-inoculation with mycorrhiza fungus and MAB, and these findings have a large impact on establishment of sapota seedlings and germplasm maintenance. |