Record ID No. |
460 |
Author(s) |
Garcia-Rodriguez J.L., Perez-Moreno J., Aldrete A., Cetina-Alcala V.M., Vaquera-Huerta H. , 2006 |
Affiliation |
Colegio Postgrad, Campus Montecillo,Km 36-5,Carretera Mexico Texco, Montecillo 56230, Texcoco, Mexico |
Title |
Characterization of the wild ectomycorrhizal fungus Pisolithus tinctorius (Pers.) Coker et Couch in culture and in symbiosis with eucalypt and pine |
Source. Vol.(no):Page |
Agrociencia. 40(5): 665-676p. |
Categories |
Ectomycorrhiza |
Subjects |
Systematics |
Sub-subjects |
Cultural Studies |
Host |
Eucalyptus urophylla, Pinus greggii |
Organism |
Pisolithus tinctorius |
Country |
Mexico, Central America |
Abstracts |
Pisolithus tinctorius is an ectomycorrhizal fungus, edible in early stages, of great relevance in the inoculation of plants with forestal importance. Three strains of fungus were isolated from fruit bodies collected in tropical eucalyptus and pine forest plantations. An in vitro characterization of these strains was carried out on three solid media at 28 degrees C. Mycelial growth in modified Melin Norkrans (MNM) liquid medium and on a vermiculite-peat mixture was assessed for all strains. In all cases, assessments were conducted at pH 4.7 and 5.7. In solid medium, greater growth in diameter and increase in biomass were observed on potato-dextrose-agar and MNM than on malt extract. The greatest increases in biomass in liquid culture medium were recorded for the strain from Oaxaca at pH 5.7. In the liquid culture, darkening of the original color of the medium was observed, as revealed by an analysis of absorbance in a mass spectrometer. Also, a morphological characterization of ectomycorrhiza synthesized with Eucalyptus urophylla and Pinus greggii was made. Because of variations in growth of the EMC fungi under different culture conditions, the characterization of ECM strains is compulsory before commercial scale production of ECM inoculum to be used in forest nurseries. |