Record ID No. |
4677 |
Author(s) |
Deepti Barnawal, Deepamala Maji, Nidhi Bharti, Chandan Singh Chanotiya, *Alok Kalra , 2013 |
Affiliation |
Microbial Technology and Entomology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, 226015, Uttar Pradesh, India, *Email: alok.kalra@yahoo.com |
Title |
ACC Deaminase-Containing Bacillus subtilis Reduces Stress Ethylene-Induced Damage and Improves Mycorrhizal Colonization and Rhizobial Nodulation in Trigonella foenum-graecum Under Drought Stress |
Source. Vol.(no):Page |
Journal of Plant Growth Regulation 32(4): 809-822p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biological Interaction |
Sub-subjects |
Plant growth promoting rhizo bacteria |
Host |
Trigonella |
Organism |
Ensifer meliloti (Em), Rhizophagus irregularis (Ri), Bacillus subtilis |
Country |
India, South Asia |
Abstracts |
This study was aimed at protecting Trigonella plants by reducing stress ethylene levels through ACC (1-aminocyclopropane-1-carboxylic acid) deaminase-containing Bacillus subtilis (LDR2) and promoting plant growth through improved colonization of beneficial microbes like Ensifer meliloti (Em) and Rhizophagus irregularis (Ri) under drought stress. A plant growth-promoting rhizobacterium strain possessing high levels of ACC deaminase characterized as B. subtilis was selected. Application of this strain considerably protected Trigonella plants under severe drought stress conditions; this protection was correlated with reduced levels of ACC (responsible for generation of stress ethylene). The experiment consisted of eight inoculation treatments with different combinations of ACC deaminase-containing rhizobacteria LDR2, Ri, and Em under three water regimes. The tripartite combination of LDR2 + Ri + Em acted synergistically to induce protective mechanisms against decreased soil water availability in Trigonella plants and improved plant weight by 56 % with lower ACC concentration (39 % less than stressed noninoculated plants) under severe drought conditions. Drought-induced changes in biochemical markers like reduced chlorophyll concentration, increased proline content, and higher lipid peroxidation were monitored and clearly indicated the protective effects of LDR2 under drought stress. Under drought conditions, apart from alleviating ethylene-induced damage, LDR2 enhanced nodulation and arbuscular mycorrhizal fungi colonization in the plants resulting in improved nutrient uptake and plant growth. |