Record ID No. |
474 |
Author(s) |
Haselwandter K., Passler V., Reiter S., Schmid DG., Nicholson G., Hentschel P., Albert K., Winkelmann G. , 2006 |
Affiliation |
University of Tubingen, Institute of Microbiology, Morgenstelle 18, D-72076 Tubingen, Germany |
Title |
Basidiochrome - a novel siderophore of the orchidaceous mycorrhizal fungi Ceratobasidium and Rhizoctonia spp. |
Source. Vol.(no):Page |
Biometals. 19(3):335-343p. |
Categories |
Orchid Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Miscellaneous |
Host |
n.a. |
Organism |
Ceratobasidium, Rhizoctonia |
Country |
Germany, Europe |
Abstracts |
A novel trishydroxamate siderophore, named basidiochrome, was isolated as the principal siderophore from low-iron culture filtrates of Ceratobasidium and Rhizoctonia species which are known as mycorrhizal fungi associated with orchid roots. Ion-exchange chromatography and preparative HPLC yielded a pure compound which contained two components according to GC-MS analysis: L-N-5-hydroxy-ornithine and 3-methyl-2-cis-pentenedioic acid (3-methyl-cis-glutaconic acid). FTICR-ESI-MS of both the iron-free and ferric form indicated an elemental composition of C33H47N6O16Fe (MW = 839) for the ferric form of basidiochrome. The connectivity was further elucidated by 2D-NMR techniques (HSQC, HMBC, COSY, NOESY) indicating that basidiochrome is a novel linear tripeptide consisting of three L-N-5-hydroxy-ornithines each linked to 3-methyl-2-cis-pentenedioic acid residues. |