Record ID No. |
4741 |
Author(s) |
Ylva Lekberg, Søren Rosendahl, Anders Michelsen, Pål Axel Olsson , 2013 |
Affiliation |
Department of Biology, University of Copenhagen, Øster Farimagsgade 2D, DK 1353, Copenhagen K, Denmark, Email: ylekberg@mpgranch.com |
Title |
Seasonal carbon allocation to arbuscular mycorrhizal fungi assessed by microscopic examination, stable isotope probing and fatty acid analysis |
Source. Vol.(no):Page |
Plant and Soil 368(1-2): 547-555p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Host |
Hypochoeris radicata |
Organism |
Arbuscular Mycorrhizal Fungi (AMF) |
Country |
Denmark, Northern Europe |
Abstracts |
Background and Aim: Climate change models are limited by lack of baseline data, in particular carbon (C) allocation to – and dynamics within – soil microbial communities. We quantified seasonal C-assimilation and allocation by plants, and assessed how well this corresponds with intraradical arbuscular mycorrhizal fungal (AMF) storage and structural lipids (16:1ω5 NLFA and PLFA, respectively), as well as microscopic assessments of AMF root colonization.
Methods: Coastal Hypochoeris radicata plants were labeled with 13CO2 in February, July and October, and 13C-allocation to fine roots and NLFA 16:1ω5, as well as overall lipid contents and AM colonization were quantified.
Results: C-allocation to fine roots and AMF storage lipids differed seasonally and mirrored plant C-assimilation, whereas AMF structural lipids and AM colonization showed no seasonal variation, and root colonization exceeded 80 % throughout the year. Molecular analyzes of the large subunit rDNA gene indicated no seasonal AMF community shifts.
Conclusions: Plants allocated C to AMF even at temperatures close to freezing, and fungal structures persisted in roots during times of low C-allocation. The lack of seasonal differences in PLFA and AM colonization indicates that NLFA analyses should be used to estimate fungal C-status. The implication of our findings for AM function is discussed. |