Record ID No. |
4894 |
Author(s) |
Beaudet D. , Terrat, Y., Halary S., De La Providencia I.E., Hijri M. , 2013 |
Affiliation |
Département de Sciences Biologiques, Institut de Recherche en Biologie Végétale, Universitéde Montréal, Montréal, QC, Canada, Email:denis.beaudet@umontreal.ca |
Title |
Mitochondrial genome rearrangements in Glomus species triggered by homologous recombination between distinct mtDNA haplotypes |
Source. Vol.(no):Page |
Genome Biology and Evolution 5(9): 1628-1643p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Genetics |
Sub-subjects |
Genetic Diversity |
Organism |
Glomus sp., G. cerebriforme |
Country |
Canada, N. America |
Abstracts |
Comparative mitochondrial genomics of arbuscular mycorrhizal fungi (AMF) provide new avenues to over come long-lasting obstacles that have hampered studies aimed at understanding the community structure, diversity, and evolution of these multinucleated and genetically polymorphic organisms. AMF mitochondrial (mt) genomes are homogeneous within isolates, and their intergenic regions harbor numerous mobile elements that have rapidly diverged, including homing end onuclease genes, small inverted repeats, and plasmid-related DNA polymerase genes (dpo), making them suitable targets for the development of reliable strain-specific markers. However, these elements may also lead to genome rearrangements through homologous recombination, although this has never previously been reported in this group of obligate symbiotic fungi. To investigate whether such rearrangements are present and caused by mobile elements in AMF, the mitochondrial genomes from two Glomeraceae members (i.e., Glomus cerebriforme and Glomus sp.) with substantialmt DNA synteny divergence,were sequenced and compared with available glomeromycotanmitochondrialgenomes. We usedan extensivenucleotide/protein similarity network-based approach to investigatedpodiversity inAMFaswell as in other organisms for which sequences are publicly available. We provide strong evidence of dpo-induced inter-haplotype recombination, leading to a reshuffled mitochondrial genome in Glomus sp. These findings raise questions as to whether AMF single spore cultivations artificially underestimatemtDNA genetic diversity.We assessed potential dpo dispersalmechanisms in AMF and inferred a robust phylogenetic relationship with plantmitochondrial plasmids. Along with other indirect evidence, our analyses indicate that members of the Glomeromycota phylum are potential donors of mitochondrial plasmids to plants. |