Record ID No. |
507 |
Author(s) |
Kuwada K., Kuramoto M., Utamura M., Matsushita I., Ishii T. , 2006 |
Affiliation |
Yamaki Company Ltd, Research Lab, Iyo 7993194, Japan |
Title |
Isolation and structural elucidation of a growth stimulant for arbuscular mycorrhizal fungus from Laminaria japonica Areschoug |
Source. Vol.(no):Page |
Journal of applied phycology. 18(6): 795-800p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Physiology |
Host |
Laminaria japonica |
Organism |
Gigaspora margarita |
Country |
Japan, Asia |
Abstracts |
Arbuscular Mycorrhizal (AM) fungi enhance terrestrial plant growth by forming a symbiotic relationship with the roots of its host plant. A growth stimulant for AM fungi was isolated from a brown alga Laminaria japonica Areschoug. The active substance for in vitro AM hyphal growth was isolated from 75% methanol extracts of L. japonica using a succession of chromatographic procedures, including flash chromatography equipped with an octa decyl silane (ODS) column, gel filtration chromatography and HPLC using an ODS column. Spores of Gigaspora margarita Becker & Hall, an AM fungus, were exposed to the compound in vitro, and hyphal growth of G. margarita was measured after two weeks of incubation. At 40 mg L-1, the compound significantly stimulated the in vitro hyphal growth of G. margarita, compared to the control. This compound was elucidated as 5'-deoxy-5'-methylamino-adenosine by EIMS and NMR spectrum. |