Record ID No. |
5197 |
Author(s) |
Jr-Hau Jiang; Yung-I Lee; Marc A. Cubeta; Lung-Chung Chen , 2015 |
Affiliation |
Department of Plant Pathology, National Chung Hsing University, No. 250, Kuo-Kuang Road, Taichung, Taiwan, e-mail: lcchen5a08@gmail.com |
Title |
Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) |
Source. Vol.(no):Page |
Mycorrhiza 25:431–445 |
Categories |
Ectomycorrhiza Ectendo Mycorrhiza Mycorrhiza General |
Subjects |
General |
Country |
Taiwan |
Abstracts |
The medicinal effects and techniques for cultivating Anoectochilus formosanus are well-documented, but little is known about the mycorrhizal fungi associated with A. formosanus. Rhizoctonia (Thanatephorus) anastomosis group 6 (AG-6) was the most common species isolated from fungal pelotons in native A. formosanus and represented 67% of the sample. Rhizoctonia (Ceratobasidium) AG-G, P, and R were also isolated and represent the first occurrence in the Orchidaceae. Isolates of AG-6, AG-R, and AG-P in clade I increased seed germination 44–91%and promoted protocorm growth from phases III to VI compared to asymbiotic treatments and isolates of AG-G in clade II and Tulasnella species in clade III. All isolates in clades I to III formed fungal pelotons in tissue-cultured seedlings of A. formosanus, which exhibited significantly greater growth than nonmycorrhizal seedlings. An analysis of the relative effect of treatment (bpi) showed that the low level of colonization (bpi ¼ 0:30–0:47) by isolates in clade I resulted in a significant increase in seedling growth compared to isolates in clades II (0.63– 0.82) and III (0.63–0.75). There was also a negative correlation (r=−0.8801) with fresh plant weight and fungal colonization. Our results suggest that isolates in clade I may represent an important group associated with native populations of A. formosanus and can vary in their ability to establish a symbiotic association with A. formosanus. The results presented here are potentially useful for advancing research on the medicinal properties, production, and conservation of A. formosanus in diverse ecosystems. |