Record ID No. |
5600 |
Author(s) |
Vangelisti A.*, Natali L., Bernadi R., Sbrana C., Turrini A., Hassani-Pak K., Hughes D., Cavallini A., Giovanneti M. and Giordani T. , 2018 |
Affiliation |
*Department of Agriculture, Food, and Environment, University of Pisa, Via del Borghetto 80, I-56124, Pisa, Italy. |
Title |
Transcriptome changes induced by arbuscular mycorrhizal fungi in sunflower (Helianthus annuus L.) roots. |
Source. Vol.(no):Page |
Scientific Reports. 8(4), doi: 10.1038/s41598-017-18445-0 |
Categories |
|
Subjects |
Genetics |
Host |
Helianthus annuus |
Organism |
Rhizoglomus irregulare |
Country |
Italy |
Abstracts |
Arbuscular mycorrhizal (AM) fungi are essential elements of soil fertility, plant nutrition and
productivity, facilitating soil mineral nutrient uptake. Helianthus annuus is a non-model, widely
cultivated species. Here we used an RNA-seq approach for evaluating gene expression variation at early
and late stages of mycorrhizal establishment in sunfower roots colonized by the arbuscular fungus
Rhizoglomus irregulare. mRNA was isolated from roots of plantlets at 4 and 16 days after inoculation
with the fungus. cDNA libraries were built and sequenced with Illumina technology. Diferential
expression analysis was performed between control and inoculated plants. Overall 726 diferentially
expressed genes (DEGs) between inoculated and control plants were retrieved. The number of upregulated DEGs greatly exceeded the number of down-regulated DEGs and this diference increased in
later stages of colonization. Several DEGs were specifcally involved in known mycorrhizal processes,
such as membrane transport, cell wall shaping, and other. We also found previously unidentifed
mycorrhizal-induced transcripts. The most important DEGs were carefully described in order to
hypothesize their roles in AM symbiosis. Our data add a valuable contribution for deciphering biological
processes related to benefcial fungi and plant symbiosis, adding an Asteraceae, non-model species for
future comparative functional genomics studies. |