Record ID No. |
5601 |
Author(s) |
Crossay T.*, Antheaume C., Redecker D., Bon L., Chedri N., Richert C., Guentas L., Cavaloc Y. and Amir Hamid. , 2017 |
Affiliation |
*Institut des Sciences Exactes et Appliquées (EA 7484), Université de Nouvelle-Calédonie, BP R4, 98851, Nouméa, Nouvelle-Calédonie, France. |
Title |
New Method for the Identification of Arbuscular mycorrhizal fungi by proteomic-based biotyping of spores using MALDI-TOF-MS. |
Source. Vol.(no):Page |
Scientific Reports. 7(14306), doi: 10.1038/s41598-017-14487-6 |
Categories |
|
Subjects |
Methodology |
Sub-subjects |
Genetic Diversity |
Host |
NA |
Organism |
NA |
Country |
France |
Abstracts |
Arbuscular mycorrhizal fungi (AMF, Glomeromycota) are mutualistic symbionts associated with
majority of land plants. These fungi play an important role in plant growth, but their taxonomic
identifcation remains a challenge for academic research, culture collections and inoculum producers
who need to certify their products. Identifcation of these fungi was traditionally performed based on
their spore morphology. DNA sequence data have successfully been used to study the evolutionary
relationships of AMF, develop molecular identifcation tools and assess their diversity in the
environment. However, these methods require considerable expertise and are not well-adapted for
“routine” quality control of culture collections and inoculum production. Here, we show that MatrixAssisted Laser Desorption Ionisation Time of Flight Mass Spectrometry proteomic-based biotyping
is a highly efcient approach for AMF identifcation. Nineteen isolates belonging to fourteen species,
seven genera and fve families were clearly diferentiated by MALDI biotyping at the species level,
and intraspecifc diferentiation was achieved for the majority. AMF identifcation by MALDI biotyping
could be highly useful, not only for research but also in agricultural and environmental applications.
Fast, accurate and inexpensive molecular mass determination and the possibility of automation make
MALDI-TOF-MS a real alternative to conventional morphological and molecular methods for AMF
identifcation. |