Record ID No. |
5603 |
Author(s) |
Ramussen S. R.*, Futchbauer W., Novero M., Volpe V., MAlkov N., Genre A., Bonfante P., Stogaard J. and Radutoiu S. , 2016 |
Affiliation |
*Department of Molecular Biology and Genetics, Centre for Carbohydrate Recognition and Signalling, Aarhus University, Denmark |
Title |
Intraradical colonization by arbuscular mycorrhizal fungi triggers induction of a lipochitooligosaccharide receptor. |
Source. Vol.(no):Page |
Scientific reports. 6(29733) doi: 10.1038/srep29733 |
Categories |
|
Subjects |
Genetics |
Sub-subjects |
Plant growth promoting rhizo bacteria |
Host |
Lotus japonicus |
Organism |
NA |
Country |
Denmark |
Abstracts |
Functional divergence of paralogs following gene duplication is one of the mechanisms leading
to evolution of novel pathways and traits. Here we show that divergence of Lys11 and Nfr5 LysM
receptor kinase paralogs of Lotus japonicus has affected their specificity for lipochitooligosaccharides
(LCOs) decorations, while the innate capacity to recognize and induce a downstream signalling after
perception of rhizobial LCOs (Nod factors) was maintained. Regardless of this conserved ability, Lys11
was found neither expressed, nor essential during nitrogen-fixing symbiosis, providing an explanation
for the determinant role of Nfr5 gene during Lotus-rhizobia interaction. Lys11 was expressed in root
cortex cells associated with intraradical colonizing arbuscular mycorrhizal fungi. Detailed analyses of
lys11 single and nfr1nfr5lys11 triple mutants revealed a functional arbuscular mycorrhizal symbiosis,
indicating that Lys11 alone, or its possible shared function with the Nod factor receptors is not essential
for the presymbiotic phases of AM symbiosis. Hence, both subfunctionalization and specialization
appear to have shaped the function of these paralogs where Lys11 acts as an AM-inducible gene,
possibly to fine-tune later stages of this interaction. |