Record ID No. |
5624 |
Author(s) |
Carotuento G.*, Sciascia I., Ludovica O., Volpe V. and Genre A. , 2019 |
Affiliation |
*Department of Life Sciences and Systems Biology, University of Turin, 10125, Torino, Italy. |
Title |
Size matters: three methods for estimating nuclear size in mycorrhizal roots of Medicago truncatula by image analysis. |
Source. Vol.(no):Page |
BMC Plant Biology. 19(180), doi: 10.1186/s12870-019-1791-1 |
Categories |
|
Subjects |
Methodology |
Host |
Medicago truncatula |
Organism |
Gigaspora margarita |
Country |
Italy |
Abstracts |
We here compare the efficiency of three Fiji/ImageJ image analysis plugins in localizing and quantifying the increase in nuclear size - a hallmark of recursive events of endoreduplication - in M. truncatula roots colonized by the AM fungus Gigaspora margarita.
All three approaches proved to be versatile and upgradeable, allowing the investigation of nuclear changes in a complex tissue; 3D Object Counter provided more detailed information than both TrackMate and Round Surface Detector plugins. |