Record ID No. |
563 |
Author(s) |
BestelCorre G., DumasGaudot E*., GianinazziPearson V ., Gianinazzi S , 2002 |
Affiliation |
*University of Bourgogne,UMR 1088,INRA,BBCE,IPM,CMSE,BP 86510,F-21065 Dijon,France |
Title |
Mycorrhiza-related chitinase and chitosanase activity isoforms in Medicago truncatula Gaertn |
Source. Vol.(no):Page |
Symbiosis, 32(3), 173-194p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Carbohydrate metabolism |
Host |
Medicago trunctatula, Checkpea |
Organism |
Glomus mosseae, sinorhizobium |
Country |
France, Europe |
Abstracts |
After inoculation with the arbuscular mycorrhizal
fungus Glomus mosseae, additional chitinase and chitosanase
isoforms; were detected in Medicago truncatula roots. Chitinase
isoforms had isoelectric points between 5.3 and 5.8 and apparent
molecular weights of 30 to 40 kDa. Western blotting demonstrated
the crossreactivity of the 30 kDa isoforms with a winged bean
class I chitinase and a class III chitinase from chickpea, while
the 40 kDa isoform only cross-reacted with the class III
chitinase from chickpea. Inoculation with other soil
microorganisms showed that these isoforms were strictly induced
during the mycorrhizal process. Chitosanase isoforms had
apparent molecular weights of 18 to 21 kDa. The three basic
isoforms could be detected in nodules isolated from
Sinorhizobium meliloti-inoculated plants, whereas the two acidic
isoforms were strictly induced during mycorrhization.
Elicitation with various chitooligosaccharides neither induced
any of the mycorrhiza-related chitinase and chitosanase
isoforms; nor had an effect on mycorrhizal infection, after
subsequent transplantation of elicited plants into Pots
containing G. Mosseae inoculum. A hypothetical role of
chitinases and chitosanases in the formation and/or degradation
of arbuscules, is discussed.
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