Record ID No. |
579 |
Author(s) |
Ezawa, T., cavagnaro, T.R., Smith, S.E., Smith, F.A., Ohyomo, R , 2004 |
Affiliation |
Nagoya University, Grad Sch Bioagr Sci, Togo Cho, Aichi 4700151, Japan. |
Title |
Rapid accumulation of polyphosphate in extraradical hyphae of an arbuscular mycorrhizal fungus as revealed by histochemistry and a polyphosphate kinase/luciferase system |
Source. Vol.(no):Page |
New Phytologist, 161(2): 387-392p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Phosphorus metabolism |
Host |
Tagetes Patulacv |
Organism |
Archaeospora leptotichaand |
Country |
Japan, Asia |
Abstracts |
The rate of polyphosphate accumulation in extraradical hyphae of an arbuscular mycorrhizal fungus was investigated by conventional histochemistry and a new enzymatic method using abacterial enzyme, polyphosphate kinase. Marigold (tagetes patulacv, Bonanza Orange)was inoculated with Archaeospora leptotichaand grown under P-deficient conditions. extraradical hyphae were harvested at 0,1,3 and 24h and 1mM P-application. Polylevels were assessed by both metachromasy of Toluidine blue and polyphosphate kinase which converted polyP to ATP increased from 25 to 44% from 0 to 1h, and a maximum of 50% was reach by 3h. Polyphosphate content was doubled from 1 to 3 h after P-application (4.8-10.0 mol as Pi mg (-1)protein) at a rate of 46.4 +/- 15.1 nmol min(-1) mg (-1). The rate of polyphosphate accumulation in the hyphae was surprisingly rapid as those polyphosphate-hyper accumulating microorganisms. The enzymatic method employed in the present study allows highly specific and sensitive assessment of polyphosphate in the mycorrhizal system. |