Record ID No. |
58 |
Author(s) |
DeBellis T., Widden P. , 2006 |
Affiliation |
Concordia University, Department of Biology, GREFi, 7100 |
Title |
Diversity of the small subunit ribosomal RNA gene of the arbuscular mycorrhizal fungi colonizing Clintonia borealis from a mixed-wood boreal forest |
Source. Vol.(no):Page |
Fems microbiology ecology. 58(2-3):225-235p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Genetics |
Host |
Boreal mixed forests, Trembling aspen, Populus tremuloides, Birch, Betula papyrifera, Spruce, Picea glauca, Balsam fir, Abies balsamea, Clintonia borealis |
Organism |
Glomus |
Country |
Canada, North America |
Abstracts |
Arbuscular mycorrhizal fungi (AMF) communities in
Clintonia borealis roots from a boreal mixed forests in
northwestern Quebec were investigated. Roots were sampled from
100 m(2) plots whose overstory was dominated by either trembling
aspen (Populus tremuloides Michx.), white birch (Betula
papyrifera Marsh.), or mixed white spruce (Picea glauca (Moench)
Voss) and balsam fir (Abies balsamea (L.) Mill.).Part of the 18S
ribosomal gene of the AMF was amplified and the resulting PCR
products were cloned. Restriction analysis of the 576 resulting
clones yielded 92 different restriction patterns which were then
sequenced. Fifty-two sequences closely matched other Glomus
sequences from Genbank. Phylogenetic analysis revealed 10
different AMF sequence types, most of which clustered with other
uncultured AM sequences from plant roots from various field
sites. Compared with other AMF communities from comparable
studies, richness and diversity were higher than observed in an
arable field, but lower than seen in a tropical forest and a
temperate wetland. The AMF communities from Clintonia roots
under the different canopy types did not differ significantly
and the dominant sequence type, which clustered with AM
sequences from a variety of environments and hosts at distant
geographical locations, represented 66.9% of all the clones
analyzed
|