Record ID No. |
580 |
Author(s) |
Hobbie, E.A., Weber, N.S., Trappe, J.M., Vanklinken, G.J , 2004 |
Affiliation |
Max Plank Institute for Biogeochemistry, Postfach 100164, D-07701 Jena, Germany |
Title |
Using radiocarbon to determine the mycorrhizal status of fungi |
Source. Vol.(no):Page |
New Phytologist, 156(1): 129-136p. |
Categories |
Mycorrhiza General |
Subjects |
Methodology |
Host |
Fungi |
Organism |
Amanita, Cantharellus, Gomphidius, Pleurocybella, Lepiota and Hypholoma, Otidia, Sowerbyella, Aleuria, Clavulina, Paurocotylis, Ramaria (sensu lato) |
Country |
Germany, Europe |
Abstracts |
Measurements of 13C in fungal sporocarps are useful in assessing mycorrhizal or saprotrophic status. Because 14C measurements can indicate the age of fungal carbon (C) and mycorrhizal fungi depend closely on recent photosynthate, 14C may provide additional insight into possible mycorrhizal status.Sporocarps, needles, and litter from Woods Creek, OR, USA together with archived sporocarps were measured for 14C content by accelerator mass spectrometry.Known mycorrhizal fungi resembled current-year needles (Amanita, Cantharellus and Gomphidius) or atmospheric CO2 (Tuber) in 14C and indicated an average age of 0–2 yr for incorporated C, whereas saprotrophic genera (Pleurocybella, Lepiota and Hypholoma) were composed of C at least 10 yr old. Of genera tentatively considered mycorrhizal from previous work with 13C, only Otidia and Sowerbyella appeared mycorrhizal from 14C measurements, whereas Aleuria, Clavulina, Paurocotylis and Ramaria (sensu lato) consisted of older carbon and were presumably saprotrophic.14C clearly separated known mycorrhizal or saprotrophic fungi and indicated 13C measurements should be interpreted cautiously on species of unknown status. 14C results for needles and mycorrhizal fungi suggested that C sources other than atmospheric CO2 may contribute small amounts of C. Possible sources include storage of arbohydrates and amino acids, organic nitrogen uptake, and incorporation of soil-respired CO2 by anaplerotic or photosynthetic pathways. |