Record ID No. |
5835 |
Author(s) |
Tsuzuki S.*, Handa Y., Takeda N. and Kawaguchi M. , 2016 |
Affiliation |
*Division of Symbiotic Systems, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki 444-8585, Aichi, Japan. |
Title |
Strigolactone-Induced Putative Secreted Protein 1 is required for the establishment of symbiosis by the Arbuscular Mycorrhizal Fungus Rhizophagus irregularis. |
Source. Vol.(no):Page |
Molecular Plant-Microbe Interactions. 29(4): 277-286. |
Categories |
|
Subjects |
Biochemistry Genetics |
Host |
Medicago truncatula |
Organism |
Rhizophagus irregularis |
Country |
Japan |
Abstracts |
Arbuscular mycorrhizal (AM) symbiosis is the most widespread association between plants and fungi. To provide novel insights into the molecular mechanisms of AM symbiosis, we screened
and investigated genes of the AM fungus Rhizophagus irregularis that contribute to the infection of host plants. R. irregularis genes involved in the infection were explored by RNA-sequencing (RNA-seq) analysis. One of the identified genes was then characterized by a reverse genetic approach using host-induced gene
silencing (HIGS), which causes RNA interference in the fungus via the host plant. The RNA-seq analysis revealed that 19 genes are up-regulated by both treatment with strigolactone (SL) (a
plant symbiotic signal) and symbiosis. Eleven of the 19 genes were predicted to encode secreted proteins and, of these, SL-induced putative secreted protein 1 (SIS1) showed the largest
induction under both conditions. In hairy roots of Medicago truncatula, SIS1 expression is knocked down by HIGS, resulting in significant suppression of colonization and formation of stunted arbuscules. These results suggest that SIS1 is a putative
secreted protein that is induced in a wide spatiotemporal range including both the presymbiotic and symbiotic stages and that SIS1 positively regulates colonization of host plants byR. irregularis. |