Record ID No. |
61 |
Author(s) |
Hortal S., Pera J., Galipienso L., Parlade J. , 2006 |
Affiliation |
IRTA (Institut de Recerca i Tecnologia Agroalimentària ), Department of Proteccio Vegetal, Central Cabril S-N, Barcelona 08348, Spain |
Title |
Molecular identification of the edible ectomycorrhizal fungus Lactarius deliciosus in the symbiotic and extraradical mycelium stages |
Source. Vol.(no):Page |
Journal of biotechnology. 126(2):123-134p. |
Categories |
Ectomycorrhiza |
Subjects |
Genetics Methodology |
Host |
n.a. |
Organism |
Lactarius deliciosus |
Country |
Spain, Europe |
Abstracts |
Specific rDNA ITS amplifications, microsatellite-primed
PCR and ITS-SSCP analysis were applied to identify and
characterize pre-selected isolates of the edible ectomycorrhizal
fungus Lactarius deliciosus in different stages of the life
cycle. Sampling was performed from pure cultures, mycorrhizas and soil from experimental plots established with
nursery-inoculated pine seedlings. A newly-designed reverse
primer (LDITS2R) combined with the universal forward ITS I
allowed to perform specific amplifications of L. deliciosus from
all the samples. Microsatellite-primed PCR using the (GTG)5
oligonucleotide as a primer showed clear polymorphisms among the
different L. deliciosus isolates. The patterns of mycorrhiza
samples showed additional bands corresponding to the plant DNA.
Single strand conformation polymorphism (SSCP) analysis of the
specific rDNA ITS fragment amplified from 18 L. deliciosus
isolates showed nine clearly different patterns. Mycorrhiza and
soil samples showed coincident patterns with their respective
fungal isolates. Specific rDNA ITS amplifications had not been
previously used for SSCP analysis of ectomycorrhizas and
extraradical mycelium. This relatively simple and inexpensive
technique allows tracking L. deliciosus isolates in different
stages of the fungus development. Specific ITS-SSCP analysis is
promising in studies of the persistence of inoculated L.
deliciosus isolates and their competitiveness with native
ectomycorrhizal fungi, especially at the extraradical mycelium
stage.
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